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| Catalog Number | orb1256746 |
|---|---|
| Category | Antibodies |
| Description | C1S Antibody |
| Target | C1S |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Rat |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Liquid |
| Purification | Affinity purification |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 479-688 of human C1S (NP_958850.1). |
| UniProt ID | P09871 |
| MW | Observed: 77kDa |
| Tested applications | IF, IHC, WB |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | EDSPD2, complement C1s subcomponent, C1 esterase, Read more... |
| Research Area | Immunology & Inflammation, Signal Transduction |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

Western blot analysis of extracts of HepG2 cells, using C1S antibody (orb1256746) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using C1S antibody (orb1256746) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using C1S antibody (orb1256746) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of A-549 cells using C1S antibody (orb1256746).
Human | |
3.13-200 ng/mL | |
1.24 ng/mL |
Mouse | |
15.63-1000 ng/mL | |
6.65ng/mL |
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