c-Myc Antibody

SKU: orb699563

Description

It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: order Ab without BSA for coating
• Reactivity: Human
• Application Notes:
  Optimal dilution of the c-Myc antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: 9E11.
• Immunogen: Amino acids AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA from C-terminus of the human protein was used as the immunogen for the c-Myc antibody. The epitope has been mapped to amino acids EQKLISEEDL.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P01106