c-Myc Antibody

• Catalog Number: orb385714
• Category: Antibodies
• Description: The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MYC699
• Tested applications: ELISA, FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from the human protein were used as the immunogen for this c-Myc antibody.
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This c-Myc antibody is available for research use only.
• UniProt ID: P01106
• Storage: Store the c-Myc antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the c-Myc antibody for each application should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.