c-Kit-IN-1

SKU: orb1302944

Description

c-Kit-IN-1 (DCC-2618) is a potent small molecule inhibitor targeting c-Met and c-Kit kinases with IC50 values below 200 nM. This compound is a valuable research tool for investigating oncogenic signaling pathways in cancers such as gastrointestinal stromal tumors (GIST) in both cellular and animal models.

Research Area

Signal Transduction

Key Properties

• CAS Number: 1225278-16-9
• MW: 489.47
• Purity: 98.73% (May vary between batches)
• Formula: C₂₆H₂₁F₂N₅O₃
• SMILES: Cn1cc(cn1)-c1cc(Oc2cc(F)c(NC(=O)C3(CC3)C(=O)Nc3ccccc3)cc2F)ccn1
• Target: c-Kit,c-Met/HGFR
• Solubility: DMSO:88 mg/mL (179.79 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (6.74 mM)

Bioactivity

• Target IC50:
  c-Met:<200 nM|c-Kit:<200 nM
• In Vitro:
  DCC-2618 also inhibits KDR and PDGFR α/β (IC50s: <2 μM, <10 μM and <10 μM).
• Cell Research:
  DCC-2618 is prepared in DMSO and stored, and then diluted with the appropriate medium before use.A serial dilution of test compounds (e.g., DCC-2618) is dispensed into a 96-well black clear bottom plate. For each cell line, five thousand cells are added per well in 200 μL complete growth medium. Plates are incubated for 67 hours at 37 degrees Celsius, 5% CO2, 95% humidity. At the end of the incubation period, 40 μL of a 440 μM solution of resazurin in PBS is added to each well and incubated for an additional 5 hours at 37 degrees Celsius, 5% CO2, 95% humidity. Plates are read on a Synergy2 reader using an excitation of 540 nM and an emission of 600 nM.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

c Kit IN 1, CD117, cKit, c-Kit, cKitIN1, c-Kit-IN-1, c-Met, cMet, cMet/HGFR, c-Met/HGFR, DCC 2618, DCC2618, DCC-2618, inhibit, HGFR, Inhibitor, SCFR, PDGFR inhibitor 1

Compound Identifiers

PubChem CID

46208890