c-Jun Antibody

• Catalog Number: orb1261262
• Category: Antibodies
• Description: c-Jun Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human c-Jun (NP_002219.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 43kDa
• Target: Jun
• UniProt ID: P05412
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: JUN, AP1, AP-1, c-Jun
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using c-Jun antibody (orb1261262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human colon using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat liver using c-Jun antibody (orb1261262) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using Histone H2AX antibody (orb1255416) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using c-Jun antibody (orb1261262) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using c-Jun antibody (orb1261262) at dilution of 1:100. Blue: DAPI for nuclear staining.