C-DIM12

SKU: orb1304534

Description

C-DIM12

Research Area

Cell Biology, Metabolism Research

Key Properties

• CAS Number: 178946-89-9
• MW: 356.85
• Purity: 99.33%
• Formula: C₂₃H₁₇ClN₂
• SMILES: Clc1ccc(cc1)C(c1c[nH]c2ccccc12)c1c[nH]c2ccccc12
• Target: Apoptosis,NR4A
• Solubility: DMSO:50 mg/mL (140.11 mM);Ethanol:35.7 mg/mL (100.04 mM)

Bioactivity

• In Vivo:
  C-DIM12 has the neuroprotective activity in MPTPp-treated mice.
• In Vitro:
  C-DIM12 induces Nurr1 and DA gene expression in cell lines and primary neurons. C-DIM12 suppresses astrocyte inflammatory signaling in vitro. C-DIM12 inhibits lipopolysaccharide (LPS)–induced expression of NF-κB-regulated genes in BV-2 microglia including nitric oxide synthase (NOS2), interleukin-6 (IL-6), and chemokine (C-C motif) ligand 2 (CCL2), and the effects were attenuated by Nurr1-RNA interference. Additionally, C-DIM12 decreased NF-κB activation in NF-κB–GFP (green fluorescent protein) reporter cells and enhanced nuclear translocation of Nurr1 primary microglia. C-DIM12 decreases lipopolysaccharide-induced p65 binding to the NOS2 promoter and concurrently enhanced binding of Nurr1 to the p65-binding site. C-DIM12 also stabilized binding of the Corepressor for Repressor Element 1 Silencing Transcription Factor (CoREST) and the Nuclear Receptor Corepressor 2 (NCOR2).
• Cell Research:
  NF-κB–GFP HEK cells are exposed to 30 ng/ml of TNFα in the presence of 100 μM C-DIM12 for up to 24 hours. C-DIM12 is efficient at blocking NF-κB–GFP expression in the NF-κB–GFP HEK cells after TNFα treatment, displaying a statistically significant reduction in total GFP fluorescence per cell.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Nurr1, Inhibitor, C DIM12, Apoptosis, CDIM12, C-DIM12, C-DIM-12

Compound Identifiers

PubChem CID

5302583