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Description
Research Area
Cell Biology
Images & Validation
−| Tested Applications | FC, ICC, IHC-P, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try 1g/ml final concentration |
| Reactivity | Human, Mouse, Other, Xenopus |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 14H5 |
| Immunogen | Fusion protein, corresponding to amino acids 281-419 of Human Bub 1 |
| Target | Bub1 |
| Purification | Protein A purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS 0.1% sodium azide; |
| Concentration | 1.1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−BUB1 budding uninhibited by benzimidazoles 1 homolog antibody;BUB1 budding uninhibited by benzimidazoles 1homolog (yeast) antibody;BUB1A antibody;BUB1L antibody;Budding uninhibited by benzimidazoles 1 (yeast homolog)antibody;Budding uninhibited by benzimidazoles 1 homolog antibody;hBUB1 antibody;Mitotic checkpointserine/threonine protein kinase BUB1 antibody;Mitotic spindle checkpoint kinase antibody;Putativeserine/threonine protein kinase antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ICC
Immunocytochemistry
















