BUB1 Antibody

SKU: orb1240079

Description

BUB1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse
• Application Notes:
  Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1.The immunogen is located within amino acids 40 - 90 of Bub1.
• Target: BUB1
• Purification: Bub1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bub1 Antibody: BUB1A, BUB1L, hBUB1, BUB1A

Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 µg/mL.

Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 µg/mL.

Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

BUB1

UniProt

O43683

RefSeq:O43683