BTC Antibody (N-term)

SKU: orb29642

Description

Rabbit polyclonal antibody to OGN belongs to the small leucine-rich proteoglycan (SLRP) family. It is expressed in bone so it encodes a protein, which induces ectopic bone formation in conjunction with TGF-beta-1 or TGF-beta-2.

Research Area

Cancer Biology, Infectious Disease & Virology, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:10-50, IF/ICC: 1:10-50, FACS: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB14431
• Immunogen: Synthetic Peptide
• Target: BTC
• Molecular Weight: 19746
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Anti-RB14431 antibody

BTC Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

BTC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of BTC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

BTC Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35 ug/lane). This demonstrates the BTC antibody detected the BTC protein (arrow).

Confocal immunofluorescent analysis of BTC Antibody (N-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Quick Database Links

Gene Symbol

BTC

UniProt

P35070

RefSeq (Protein):NP_001720.1