BTB06584

SKU: orb1225641

Description

BTB06584 is a novel acetohydroxyacid synthase(AHAS) inhibitor, a promising drug target against Mycobacterium tuberculosis (MTB). BTB06584 is an IF1-dependent, selective inhibitor of the mitochondrial F1 Fo-ATPase.

Key Properties

• CAS Number: 219793-45-0
• MW: 417.82
• Purity: >98% (HPLC)
• Formula: C₁₉H₁₂ClNO₆S
• SMILES: O=S(C1=CC(OC(C2=CC=C(Cl)C=C2)=O)=C([N+]([O-])=O)C=C1)(C3=CC=CC=C3)=O
• Target: ATPase
• Solubility: DMSO: 84 mg/mL (201.04 mM)

Bioactivity

• In Vivo:
  BTB06584 (1 μM; 24 hours) treatment rescued defective haemoglobin synthesis in zebrafish pinotage (pnt) mutants in which expression of the Atpif1a gene is lost. The concentrations of BTB06584 that restore haemoglobin biosynthesis also alter mitochondrial bioenergetics in living fish.
• In Vitro:
  In HL-1 cells, BTB06584 (100 μM) inhibits F1Fo-ATPase activity with no effect on the mitochondrial membrane potential (ΔΨm ) or O2 consumption. BTB06584 (100 μM) pretreatmet protects against ischaemic cell death in HL-1 cells prior to a period of ischaemia. ATP consumption is decreased following inhibition of respiration, and ischaemic cell death is reduced. BTB06584 efficiency is increased by IF1 overexpression and reduced by silencing the protein.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BTB

Quick Database Links

Gene Symbol

ATPase