Brontictuzumab Antibody

SKU: orb1980658

Description

Brontictuzumab (OMP 52M5) is a Notch1-targeting monoclonal antibody that blocks pathway activation, demonstrating antitumor and anti-proliferative effects. It is utilized in research models for leukemias and lymphomas, including both in vitro cellular studies and in vivo xenograft experiments.

Research Area

Neuroscience, Protein Biochemistry, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 1447814-75-6
• MW: 143.93 kDa
• Purity: 98.1% (SDS-PAGE); 97.6% (SEC-HPLC)
• Target: Gamma-secretase

Bioactivity

• In Vivo:
  In the MCL model, 20 mg/kg Brontictuzumab intraperitoneal injection, administered every 4 days, inhibited DLL4-induced Notch1 activation. Intraperitoneal injection of 15 mg/kg Brontictuzumab significantly reduced tumor burden in a T-ALL xenograft model.
• In Vitro:
  0-100 μg/mL Brontictuzumab effectively inhibited the activity of Notch1 signaling pathways, including DLL4 and JAG1/2. In HPB-ALL cell line, treatment with 25 μg/mL Brontictuzumab for 4 days significantly reduced the level of Notch1 intracellular structural domain. In MCL cells, treatment with 25 μg/mL Brontictuzumab for 48 hours inhibited DLL4-mediated overexpression of cleaved Notch1. In addition, the increased phosphorylation of MEK and ERK in Mino cells caused by DLL4 stimulation was effectively blocked.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Human NOTCH1 Recombinant Antibody, OMP52M5, OMP 52M5, Notch, Gammasecretase

Quick Database Links

Gene Symbol

Gamma-secretase