Bromoenol lactone

SKU: orb2901619

Description

Bromoenol lactone ((6E)-Bromoenol lactone) is a suicide-based irreversible, selective, potent inhibitor of calcium-independent phospholipase A2 (iPLA2β) with an IC50 value of approximately 7 μM, which inhibits antigen-stimulated mast cell exocytosis without blocking Ca2+ influx.

Key Properties

• CAS Number: 88070-98-8
• MW: 317.18
• Purity: >98% (HPLC)
• Formula: C₁₆H₁₃BrO₂
• SMILES: O=C1C(C=2C3=C(C=CC2)C=CC=C3)CC/C(=C\Br)/O1
• Target: Phospholipase
• Solubility: In Vitro: DMSO : 50 mg/mL (157.64 mM; Ultrasonic )

Bioactivity

• In Vitro:
  In RBL 2H3 and bone marrow-derived mast cells (BMMCs), Ca2+ entry is critical for exocytosis. Bromoenol lactone inhibits exocytosis when stimulated using a Ca2+ ionophore A23187, which passively transports Ca2+ down a concentration gradient and also in permeabilised mast cells where Ca2+ entry is no longer relevant. Moreover, Bromoenol lactone has only a minor effect on antigen- or thapsigargin-stimulated Ca2+ signalling, both the release from internal stores and sustained elevation due to Ca2+ influx.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Phospholipase