Brk (phospho Tyr447) rabbit pAb

• Catalog Number: orb769791
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Brk (phospho-Tyr447)
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Breast Tumor Kinase around the phosphorylation site of Tyr447. AA range:402-451
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 50
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-PTK6 antibody, anti-BRK antibody, anti-Protei
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of 293 cells using Phospho-Brk (Y447) Polyclonal Antibody.

Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The lane on the right is blocked with the phospho peptide.