Brilliant Black BN

SKU: orb1307221

Description

Brilliant Black BN

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 2519-30-4
• MW: 867.68
• Formula: C₂₈H₁₇N₅Na₄O₁₄S₄
• SMILES: [Na+].[Na+].[Na+].[Na+].CC(=O)Nc1ccc(c2cc(c(\N=N\c3ccc(\N=N\c4ccc(cc4)S(=O)(=O)[O-])c5ccc(cc35)S(=O)(=O)[O-])c(O)c12)S(=O)(=O)[O-])S(=O)(=O)[O-]
• Target: Virus Protease
• Solubility: DMSO:123 mg/mL (141.76 mM)

Bioactivity

• Target IC50:
  EV71 (various strains):2.39 µM- 28.12 µM (IC50)
• In Vivo:
  Brilliant black BN (i.p.; 200 mg/kg; 14 days)exhibits mild illness with clinical scores less than 3 and subsequently recovered when compared with PBS-group. All E151 270 treated mice are completely protected throughout the experiment .
• In Vitro:
  Brilliant black BN inhibited the infection EV71 -GFP at a concentration of 300 μM. Brilliant black BN exhibits the inhibition of EV71 -GFP as a dose-dependent manner in infected RD cells (IC50: 10.1 μM). Brilliant black BN is able to inhibit all tested 28 EV71, CVA16, and CVA6 strains. In rhabdomyosarcoma cells, 50% inhibitory concentration 29 of the dye E151 for various strains of EV71 ranged from 2.39 μM to 28.12 μM, whereas its 50% cytotoxic concentration is 1870 μM .
• Cell Research:
  Resting cell dye decolorization experiment 1. Deep-sea isolates were transferred to fresh 250 mL-GYE broth, centrifuged and added to 20 mM Tris-HCl (pH 7.6) for resting cell assays. 2. Cells were grown in 10 mL tubes (final cell dry weight up to 5.20–5.38 g/L), then Brilliant Black BN was added to a final concentration of 100 mg/L, the tubes were gently inverted several times, and incubated at 30°C, taking care not to shake; 3. Samples (200 μL) were collected regularly from 0 to 3 hours, immediately boiled in a dry heat bath for 5 minutes, and then centrifuged (13,000 g, 10 minutes); 4. The residual dye concentration of the supernatant was measured.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

disease, E151, E-151, E 151, Brilliant Black BN, additive,contagious, antiviral,food, Inhibitor, HEVs, HEV, HFMD, HRV, HRVs, EV71,infection, Enterovirus, inhibit, various strains of EV71, VirusProtease, Virus Protease, Rhinovirus

Compound Identifiers

PubChem CID

17293