Brevican Antibody (HRP)

SKU: orb150305

Description

Mouse monoclonal to Brevican (HRP). Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE..

Research Area

Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of Brevican in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone No.: N294A/6 (Formerly sold as S294A-6)
• Immunogen: Fusion protein amino acids 219-655 of rat Brevican
• Target: Brevican
• Molecular Weight: 140kDa
• Purification: Protein G Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Brevican, BCAN, BEHAB, CSPG7, Brain-enriched hyaluronan-binding protein, Brevican core protein, Brevican core protein isoform 1, Brevican core protein isoform 2, Brevican proteoglycan, Chondroitin sulfate proteoglycan 7, Chondroitin sulfate proteoglycan BEHAB, MGC13038

Quick Database Links

Gene Symbol

Brevican

UniProt

P55068

NCBI Gene ID

25393

RefSeq (Protein):NP_001028837.1