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Brevican Antibody: APC
Catalog Number: orb150302
| Catalog Number | orb150302 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal to Brevican (APC). Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | N294A/6 (Formerly sold as S294A-6) |
| Tested applications | ICC, IF, IHC |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG2B |
| Immunogen | Fusion protein amino acids 219-655 of rat Brevican |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000) |
| Conjugation | APC |
| MW | 140kDa |
| Target | Brevican |
| Entrez | 25393 |
| UniProt ID | P55068 |
| NCBI | NP_001028837.1 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Alternative names | BCAN antibody, BEHAB antibody, CSPG7 antibody, Bra Read more... |
| Note | For research use only |
| Application notes | 1 µg/ml of SMC-428 was sufficient for detection of Brevican in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone N294A/6. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Brevican Antibody (D) Composite.
Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone N294A/6. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 μg). Load: 10 μg. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.
