BrdU Antibody / Bromodeoxyuridine

• Catalog Number: orb699496
• Category: Antibodies
• Description: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: All
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Bromodeoxyuridine conjugated to KLH was used as the immunogen for the recombinant BrdU antibody.
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Application notes: Optimal dilution of the recombinant BrdU antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rBRD494
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Store the recombinant BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This recombinant BrdU antibody is available for research use only.
• Note: For research use only

SDS-PAGE analysis of purified, BSA-free recombinant BrdU antibody (clone rBRD494) as confirmation of integrity and purity.