BRD4 Antibody (C-term)

SKU: orb1928649

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: IHC-P - 1:50-100
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD5644
• Immunogen: This BRD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1313~1342 amino acids from the C-terminal region of human BRD4. Antigen Region: 1313-1342 aa.
• Target: BRD4
• Molecular Weight: 152219 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bromodomain-containing protein 4, Protein HUNK1, BRD4, HUNK1

Subnuclear distribution of cellular proteins. CHOBgl40 cells grown on coverslips were either directly or after treatment with 0.5% Triton X-100, incubated with antibodies against Brd4. PI, propidium iodide staining of cellular DNA.WC, whole cells.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

BRD4

UniProt

O60885

RefSeq (Protein):NP_055114.1, NP_490597.1