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BRAF V600E Antibody(C-Term)
Catalog Number: orb1925400
| Catalog Number | orb1925400 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB56427 |
| Tested applications | IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:2000, IHC-P: 1:25 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 84437 Da |
| Target | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 576-606 amino acids from human. |
| UniProt ID | P15056 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Serine/threonine-protein kinase B-raf, 2.7.11.1, P Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Staining BRAF in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes: Anti-BRAF Antibody (C-Term) at 1:2000 dilution. Lane 1: Braf-600V -wild type. Lane 2: Braf-600E -mutation. Lane 3: Raji whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: A549 whole cell lysate. Lane 6: T47D whole cell lysate. Lane 7: MDA-MB-453 whole cell lysate. Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
