BRAF Antibody

SKU: orb1939425

Description

BRAF Antibody

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Immunology & inflammation, Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB - 1:500-2000, IF - 1:10-50
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1κ
• Molecular Weight: 84437 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BRAF1, RAFB1

All lanes: Anti-BRAF Antibody at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: K562 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of C2C12 cell stained with BRAF Antibody. C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37°C. For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C.BRAF immunoreactivity is localized to Cytoplasm significantly.

Western blot analysis of anti-BRAF Antibody in HL-60 cell line lysates (35 μg/lane). BRAF (arrow) was detected using the purified Mab.

Quick Database Links

UniProt

P15056