Bovine IgG (H&L) Antibody Texas Red Conjugated

SKU: orb346816

Description

Bovine IgG (H&L) antibody (Texas Red)

Images & Validation

• Tested Applications: FC, FLISA, IF, WB
• Dilution Range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Bovine
• Application Notes:
  Anti-Bovine IgG Antibody Texas Red Conjugated has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Bovine IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Bovine IgG and Bovine Serum.
• Conjugation: Texas Red

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000); Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Goat anti Bovine IgG Texas Red Conjugated, Goat anti-Bovine IgG Texas Red Conjugated

Western Blot of Goat anti-Bovine IgG Texas Red Conjugated Secondary Antibody. Lane 1: Bovine IgG. Lane 2: None. Load: 100 ng per lane. Primary antibody: None. Secondary antibody: Texas Red goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Bovine IgG. Other band(s): Bovine IgG splice variants and isoforms.