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Bovine IgG (H&L) Antibody Peroxidase Conjugated

SKU: orb420149

Description

Bovine IgG (H&L) antibody (Peroxidase)

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:5,000 - 1:100,000, IHC: 1:500 - 1:5,000, WB: 1:5,000 - 1:200,000
ReactivityBovine
Application Notes
This antibody is supplied in 0.01M Sodium Phosphate, 0.25M NaCL, pH: 7.6. Anti-Bovine IgG (heavy and light chain) Antibody is peroxidase conjugated and is suitable for immunoassays where specificity to bovine immunoglobulin is desired. Antibody has been tested by ELISA, western blot immunoblot, and immunohistochemistry. Optimal concentrations in immunoassays should be determined by the researcher.

Key Properties

Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenBovine IgG whole molecule
PurityAnti-Bovine IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Bovine IgG and Bovine Serum.
ConjugationHRP

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: None. Do NOT add sodium azide!. Stabilizer: 15 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: See application note.
Concentration0.8 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

goat anti-Bovine IgG peroxidase Conjugated Antibody, goat anti-Bovine IgG HRP Conjugated Antibody

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Bovine IgG (H&L) Antibody Peroxidase Conjugated (orb420149)

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2 ml
$ 410.00
DispatchUsually dispatched within 5-10 working days
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