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Catalog Number | orb346822 |
---|---|
Category | Antibodies |
Description | Bovine IgG F(c) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine |
Isotype | IgG |
Immunogen | Anti-Bovine IgG F(c) was produced by repeated immunization with bovine IgG F(c) fragment in rabbit. |
Concentration | 2.0 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Bovine IgG, Bovine IgG F(c) and Bovine Serum. No reaction was observed against Bovine IgG F(ab). |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Bovine IgG F(c) Antibody, Rb-a-Bovine Read more... |
Note | For research use only |
Application notes | Anti-Bovine IgG F(c) Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. |
Expiration Date | 12 months from date of receipt. |
Texas Red | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine IgG, anti-Bovine IgG F(c) and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(ab’)2 or anti-Papain. | |
Bovine |
DOT, SDS-PAGE | |
Unconjugated | |
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum, anti-Bovine IgG and anti-Bovine IgG F(c). No reaction was observed against anti-Bovine IgG F(ab’)2 or anti-Papain. | |
Bovine |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Bovine IgG, anti-Bovine IgG F(c) and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(ab’)2 or anti-Papain. | |
Bovine |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Bovine IgG, anti-Bovine IgG F(c) and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(ab’)2 or anti-Papain. | |
Bovine |
Biotin | |
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Bovine IgG, anti-Bovine IgG F(c) and anti-Bovine Serum. No reaction was observed against anti-Bovine IgG F(ab’)2 or anti-Papain. | |
Bovine |
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