BNIP3 Antibody

• Catalog Number: orb1257387
• Category: Antibodies
• Description: BNIP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human BNIP3 (NP_004043.3).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 22-70kDa
• Target: BNIP3
• UniProt ID: Q12983
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: BCL2/adenovirus E1B 19 kDa protein-interacting pro
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using BNIP3 antibody (orb1257387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded mouse kidney using BNIP3 antibody (orb1257387) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody (orb1257387) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver damage using BNIP3 antibody (orb1257387) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using BNIP3 antibody (orb1257387) at dilution of 1:100. Blue: DAPI for nuclear staining.