BMP4 Antibody

SKU: orb1272672

Description

BMP4 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Human
• Application Notes:
  Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hBMP-4 (5.0 ng/mL), a concentration of 0.4-0.6 μg/mL of this antibody is required.ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human BMP-4 when used in conjunction with compatible secondary reagents. Western Blot:To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Clone No.: 3H2
• Immunogen: BMP-4 antibody is produced in mice using highly pure (>98%) recombinant human BMP-4 as the immunizing antigen.
• Target: BMP4
• Purification: This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bone morphogenetic protein, BMP2B, DVR4, Bone morphogenetic protein 4, Bone morphogenetic protein 2B, BMP-4, ZYME, OFC11, BMP2B1, MCOPS6

This antibody stained HepG2 cells. The primary antibody was incubated at 3.0 ug/ml overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

BMP4

UniProt

P12644

RefSeq:P12644