BMI1 Antibody

SKU: orb1939348

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: B404EV5X9X8
• Immunogen: This BMI1 monoclonal antibody is generated from mouse immunized with BMI1 recombinant protein.
• Target: BMI1
• Molecular Weight: 36949 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, BMI1, PCGF4, RNF51

All lanes: Anti-BMI1 Antibody at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: A549 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 37 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

BMI1 Antibody western blot analysis in K562 cell line lysates (35 μg/lane). This demonstrates the BMI1 antibody detected the BMI1 protein (arrow).

Confocal immunofluorescent analysis of BMI1 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Quick Database Links

Gene Symbol

BMI1

UniProt

P35226

RefSeq (Protein):NP_001190991.1, NP_005171.4