BMI1 Antibody (C-term)

SKU: orb1931590

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB02926
• Immunogen: This BMI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the C-terminal region of human BMI1. Antigen Region: 257-286 aa.
• Target: BMI1
• Molecular Weight: 36949 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, BMI1, PCGF4, RNF51

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with BMI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of BMI1 Antibody (C-term) antibody in 293 cell line lysates (35 ug/lane). BMI1 (arrow) was detected using the purified Pab.

BMI1 Antibody (C-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the BMI1 antibody detected the BMI1 protein (arrow).

Quick Database Links

Gene Symbol

BMI1

UniProt

P35226

RefSeq (Protein):NP_001190991.1, NP_005171.4