BMAL1 Recombinant Rabbit Monoclonal Antibody

SKU: orb1499364

Description

BMAL1 Recombinant Rabbit Monoclonal Antibody

Research Area

CARdiovascular Research, Cardiovascular Research, Cholesterol Metabolism, DNA and RNA Research, Epigenetics and Nuclear Signaling, Metabolism, Transcription Factors

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1:50-100
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: B0F3
• Immunogen: KLH conjugated synthetic peptide derived from human BMAL1
• Target: BMAL1
• Molecular Weight: 73-75 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARNTL; ARNTL1; BMAL1c; JAP3; MOP3; PASD3; TIC; bHLHe5; Arnt3; BMAL1b; bmal1b'; BMAL1_HUMAN; BMAL1; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic-helix-loop-helix-PAS protein MOP3; Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5 (bHLHe5); Member of PAS protein 3; PAS domain-containing protein 3; bHLH-PAS protein JAP3; BMAL1_MOUSE; BMAL1_RAT;

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: PC-3, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 68 kDa, Observed MW: 75 kDa.

Cell line: SH-SY5Y, Fixation: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary Ab Dilution: 1:100, Secondary Ab: Goat Anti-Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1499364.

ICC staining of BMAL1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1499364, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).

Tissue: Mouse hippocampus, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499364.

Tissue: Mouse pancreas, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499364.

Western blot analysis of BMAL1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb1499364, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, Lane 2: NIH/3T3 cell lysate, Lane 3: Rat brain tissue lysate, Lane 4: Mouse spleen tissue lysate.

Quick Database Links

Gene Symbol

BMAL1

UniProt

O00327