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BLZF1 Antibody (Center)
Description
Research Area
Images & Validation
−| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF: 1:10~50, WB: 1:1000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB21531 |
| Target | This BLZF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the Central region of human BLZF1. |
| Molecular Weight | 44910 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of BLZF1 Antibody (Center) in HepG2 cell line lysates (35 ug/lane). BLZF1 (arrow) was detected using the purified Pab.

Fluorescent image of A549 cell stained with BLZF1 Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BLZF1 primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).BLZF1 immunoreactivity is localized to Nucleus significantly.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_003657.1 |
|---|
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Protocol Information
BLZF1 Antibody (Center) (orb1927837)
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