BLZF1 Antibody (Center)

• Catalog Number: orb1927837
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This BLZF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the Central region of human BLZF1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9H2G9
• MW: 44910 Da
• Tested applications: IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000
• Antibody Type: Primary Antibody
• Clone Number: RB21531
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Golgin-45, Basic leucine zipper nuclear factor 1,
• Research Area: Cell Biology, Infectious Disease & Virology, Signa
• Note: For research use only
• NCBI: NP_003657.1

Western blot analysis of BLZF1 Antibody (Center) in HepG2 cell line lysates (35 ug/lane). BLZF1 (arrow) was detected using the purified Pab.

Fluorescent image of A549 cell stained with BLZF1 Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BLZF1 primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).BLZF1 immunoreactivity is localized to Nucleus significantly.