(-)-Blebbistatin

SKU: orb1301129

Description

(-)-Blebbistatin is the potent and selective S-enantiomeric inhibitor of myosin II isoforms, exhibiting IC50 values in the low micromolar range. It is widely employed in vitro to study cytoskeletal dynamics, cell division, and migration, and has been used in vivo to investigate cardiac function and muscle contraction mechanisms.

Research Area

Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 856925-71-8
• MW: 292.33
• Purity: 99.61%
• Formula: C₁₈H₁₆N₂O₂
• SMILES: Cc1ccc2N=C3N(CC[C@@]3(O)C(=O)c2c1)c1ccccc1
• Target: ATPase,Myosin
• Solubility: DMSO:105 mg/mL (359.18 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2.93 mg/mL (10.02 mM)

Bioactivity

• Target IC50:
  non muscle myosin II ATPase (cell-free assays):2 μM|Smooth muscle myosin:80 μM|Non-muscle myosin II ATPases:0.5 μM-5 μM
• In Vivo:
  Blebbistatin completely relaxes both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction in a dose-dependent manner. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz.
• In Vitro:
  METHODS: Mouse hepatic stellate cell HSCs were treated with (-)-Blebbistatin (12.5-50 µM) for 2 h and cell morphology was observed using microscopy. RESULTS: When treated with (-)-Blebbistatin for 2 h, the cells became smaller, acquired more dendritic morphology, and had fewer stress fibers and lamellipods. METHODS: Porcine TM cells were treated with (-)-Blebbistatin (25-100 µM) for 2 h. The expression levels of target proteins were detected using Western Blot. RESULTS: There was no difference in MLC phosphorylation status between control and drug-treated samples.
• Cell Research:
  Freshly isolated HSCs are replated on 96-well plate. At day 3, medium is replaced by serum-free medium and cells are starved overnight, treated with or without blebbistatin (25 μM) for 2 h followed by stimulation with platelet-derived growth factor-BB (20 ng/mL). After an overnight incubation, the WST-1 cell proliferation assay are performed.

Storage & Handling

• Storage: store at low temperature,keep away from moisture | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, non-muscle myosin II ATPases, Myosin, inhibit, Blebbistatin, (S)-(-)-Blebbistatin, ()Blebbistatin, ( ) Blebbistatin

Compound Identifiers

PubChem CID

5287792