Bisindolylmaleimide VIII

SKU: orb1738355

Description

Bisindolylmaleimide VIII (Ro-31-7549) is a potent, selective PKC inhibitor effective against multiple isoforms. It suppresses T cell-mediated autoimmunity and potentiates Fas-induced apoptosis via PKC-independent mechanisms, making it a valuable tool for studying immune disorders and apoptosis in cellular and animal models.

Research Area

Cell Biology, Epigenetics & Chromatin, Signal Transduction

Key Properties

• CAS Number: 125313-65-7
• MW: 398.46
• Purity: 97.00% (May vary between batches)
• Formula: C₂₄H₂₂N₄O₂
• SMILES: O=C1NC(=O)C(C2=CN(C=3C=CC=CC23)CCCN)=C1C4=CN(C=5C=CC=CC45)C
• Target: PKC,Apoptosis

Bioactivity

• Target IC50:
  PKCβII:163 nM|PKCγ:213 nM|PKCε:175 nM|PKCβI:195 nM|PKCα:53 nM|PKC (rat brain):158 nM
• In Vivo:
  Ro-31-7549 (100 μg; i.p.; every other day for three doses; 6-8-week-old female NOD/SCID mice) combined with toTRA-8, resulted in nearly complete tumor regression. Bisindolylmaleimide VIII alone did not result in significant tumor regression.
• In Vitro:
  Ro-31-7549 (5 μM; 8, 12h; 1321N1 cells) significantly increases TRA-8-induced cell death in a time- and dose-dependent manner. After combined treatment with Ro-31-7549 (5 μM; 6h; 1321N1 cells) and TRA-8, procaspase-8 was significantly reduced at 4 h and completely disappeared at 6h.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis, Bis VIII, UNII-FC61260G4Q, Vortex, PKC, Ro-31-7549, Ro-31-7549 Monohydrate, Ro 31-7549, RO 318161

Compound Identifiers

PubChem CID

2403