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GPRC5D Antibody
Description
Images & Validation
−| Tested Applications | ELISA, IHC |
|---|---|
| Dilution Range | ELISA 1/5000-10000|Flow Cyt 1/100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | DM61 |
| Target | GPRC5D |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Powder |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−GPRC5D rabbit pAb Antibody [orb769604]
ELISA, IF, WB
Human, Mouse
Polyclonal
Unconjugated
100 μl, 50 μlGPRC5D Antibody [orb757384]
ELISA, IHC
Human
Rabbit
Monoclonal
Unconjugated
500 μg, 100 μg, 50 μg, 10 μg

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ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human GPRC5D, mFc tagged protein can bind Biotinylated Anti-GPRC5D antibody in a linear range of 0.488-2000 ng/ml

Immunohistochemical analysis of paraffin-embedded RPMI8226 transplanted tumor labeling biotinylated Anti-GPRC5D antibody (Cat#orb1173962) at 10 μg/ml, followed by Streptavidin (HRP) ready to use. Membranous staining on RPMI8226 cells. Counter stained with Hematoxylin.Control: Used Anti-GPRC5D antibody (Cat#orb757384) instead of primary antibody, secondary antibody is Streptavidin (HRP) Ready to use.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

orb1173962 at 10 μg/ml staining GPRC5D in in human tonsil tissue by IHC (SKU# orb1173962)
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Protocol Information
GPRC5D Antibody (orb1173962)
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