Recombinant Biotin Antibody / Rabbit Monoclonal

SKU: orb2640787

Description

This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application Notes:
  The optimal dilution of the recombinant Biotin antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: BTN/2032R
• Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this recombinant Biotin antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Kappa Light Chain Ab probe followed by anti-Biotin antibody (clone BTN/2032R). No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

SDS-PAGE analysis of purified, BSA-free recombinant Biotin antibody (clone BTN/2032R) as confirmation of integrity and purity.