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Catalog Number | orb64052 |
---|---|
Category | Tools |
Description | Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine. |
Concentration | 1.0 mM-1.1 mM |
Form/Appearance | filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5 |
Purity | ≥ 95% (HPLC) |
MW | Theoretical MW: 882.71 g/mol (free acid); Detected MW: 882.19 g/mol (free acid) |
SMILES | N1C(=O)N[C@@H]2[C@H]1[C@H](CCCCC(=O)NCCCCCC(=O)NCC#Cc1cn(c3ncnc(N)c13)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)SC2 |
Formula | C30H45N8O15P3S |
Storage | store at -20 °C |
Note | For research use only |
Application notes | Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; Reverse Transcription with MMLV Reverse Transcriptase.
Spectroscopic Propertie: λmax 280 nm, ε 12.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
Expiration Date | 12 months from date of receipt. |
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