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Biotin-14-dATP
Catalog Number: orb533181
| Catalog Number | orb533181 |
|---|---|
| Category | Small Molecules |
| Description | Biotin-14-dATP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N6 position of adenine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dATP is recommended whose Biotin moiety is attached to the N7-Deaza position of adenine via a 11-atom linker. Recommended Biotin-14-dATP/dATP ratio for Nick Translation: 50% Biotin-14-dATP/50% dATP. |
| Form/Appearance | filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5 |
| Concentration | 1.0 mM-1.1 mM |
| Purity | ≥ 95% (HPLC) |
| MW | Theoretical MW: 929.81 g/mol (free acid); Detected MW: 929.27 g/mol (free acid) |
| Application notes | Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow fragment. Spectroscopic Propertie: λmax 266 nm, ε 16.2 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
| Formula | C32H54N9O15P3S |
| SMILES | OP(=O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]1O[C@@H](n2cnc3c(NCCCCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]4SC[C@@H]5NC(=O)N[C@H]45)ncnc23)C[C@@H]1O |
| Storage | store at -20 °C |
| Hazard Information | Non-Toxic |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Structural formula of Biotin-14-dATP.
For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.Note:
- For Reference Only. Please choose the appropriate dissolution protocol according to your experimental animal and administration method.
- We suggest that in cell experiments, the final concentration of DMSO is not more than 0.1%, in animal experiments, for healthy mice, the final concentration of DMSO is not more than 10%; For nude or frail mice, the final concentration of DMSO is not more than 2%.
