BIO-acetoxime

SKU: orb1300466

Description

BIO-acetoxime (GSK-3 Inhibitor X) is a highly potent and selective dual inhibitor of GSK3α and GSK3β, exhibiting an IC50 of 10 nM. It demonstrates significant selectivity, being over 240-fold more potent against GSK3 than against related kinases CDK5/p25, CDK2/cyclin A, and CDK1/cyclin B. This inhibitor is a valuable research tool for studying GSK3 signaling in neurological disorders and cellular models both in vitro and in vivo.

Research Area

Cell Biology, Infectious Disease & Virology, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 667463-85-6
• MW: 398.21
• Purity: 97.28%
• Formula: C₁₈H₁₂BrN₃O₃
• SMILES: CC(=O)O\N=C1\C(\Nc2ccccc\12)=C1\C(=O)Nc2cc(Br)ccc12
• Target: GSK-3,HSV,Apoptosis
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (2.51 mM);DMSO:8 mg/mL (20.09 mM)

Bioactivity

• Target IC50:
  CDK5-p35:2.4 μM|CDK2-CyclinA:4.3 μM|CDK1-CyclinB:63 μM|GSK-3α:10 nM|GSK-3β:10 nM
• In Vitro:
  In human oral epithelial cells, BIO-acetoxime suppresses viral gene expression and protects oral epithelial cells from HSV-1 infection. In SY5Y-MYCN cells, BIO-acetoxime strongly reduces c-MYC expression and p-SMAD3 levels. BIO-acetoxime also decreases cell viability of KCN, KCNR, SY5Y, Kelly, and IMR32 cells by mediating apoptosis. In HEK 293T cells, BIO-acetoxime is also found to reduce antiviral innate immunity downstream of IRF3 activation by inhibition of GSK3α/β activities.
• Cell Research:
  Cell viability is analyzed by CellTiter 96 AQueous One Solution Cell Proliferation Assay according to manufacturer's instructions, approximately 5,000 cells are plated per well of 96-well tissue culture plates with 100 μL of medium. To assess cell viability, rather than proliferation rate, inhibitor- and control-treated cells are assayed after the same growth time had elapsed. The results represent the mean ± SD of triplicate samples, expressed as a percentage of control.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, Herpes simplex virus, HSV, GSK-3 Inhibitor X, GSK-3α, GSK-3β, GSK3, GSK-3, Glycogen synthase kinase 3, Glycogen synthase kinase-3, inhibit, Apoptosis, BIO acetoxime, BIOacetoxime, BIO-acetoxime, BIA

Compound Identifiers

PubChem CID

135398521