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BIN1 Antibody

SKU: orb344473

Description

BIN1 antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

Tested ApplicationsELISA, IF, IP, WB
Dilution RangeELISA: 1:5000 - 1:50000, IF: 1:100-1:500, IP: 10-100 µL, WB: 1:500-1:1500
ReactivityHuman, Mouse
Application Notes
Anti-BIN1 antibody has been tested for use in ELISA, Western Blot, IP, and IF. Specific conditions for reactivity should be optimized by the end user.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG
Clone No.99F
ImmunogenAnti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with BIN1 polypeptide followed by hybridoma development.
TargetBIN1
PurityAnti-BIN1 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. BIN1 antibody is specific for human BIN1 protein. A BLAST analysis was used to suggest cross-reactivity with BIN1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN1 from other sources has not been determined.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.0 mg/ml
Expiration Date12 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

mouse anti-BIN1 Antibody, AMPHL, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, BIN 1, BIN-1, BIN1 antibody, anti-BIN1 antibody

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

Select a size below

100 μg
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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