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BLRO_MYRVE Antibody
Description
Images & Validation
−| Tested Applications | ELISA, IHC, IP, WB |
|---|---|
| Dilution Range | ELISA: 1:25,000, IHC: 1:1,000 - 1:5,000, IP: 1:100, WB: 1:2,000 - 1:10,000 |
| Reactivity | Fungi |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Immunogen | Bilirubin Oxidase (Myrothecium verrucaria) |
| Target | BLRO_MYRVE |
| Purity | This product was prepared from monospecific antiserum by delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Bilirubin Oxidase (Myrothecium verrucaria). Cross-reactivity against Bilirubin Oxidase from other sources is unknown. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 95 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Biorbyt Goat anti Bilirubin Oxidase antibody was used to detect Bilirubin Oxidase under reducing (R) conditions. A samples of ~1 ug of purified protein contained 4% BME and was boiled for 5 minutes and run by SDS-PAGE on a 4-20% acrylamide gel. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/orb348637 1 hr RT) and Dylight 649 conjugated Donkey anti goat (1:10K in TBS/orb348637) 1 hr RT.
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Protocol Information
BLRO_MYRVE Antibody (orb750400)
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