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Item 1 of 3
Bik BH3 Domain Antibody
Catalog Number: orb1936855
| Catalog Number | orb1936855 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This Bik BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-74 amino acids from human Bik BH3 Domain. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q13323 |
| MW | 18016 Da |
| Tested applications | IHC-P, WB |
| Dilution range | WB: 1:1000, WB: 1:2000, IHC-P: 1:50~100 |
| Antibody Type | Primary Antibody |
| Clone Number | RB3381 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Bcl-2-interacting killer, Apoptosis inducer NBK, B Read more... |
| Note | For research use only |
| NCBI | NP_001188.1 |
All lanes: Anti-hBik-BH3 at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Ramos whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 18 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Bik Antibody (BH3) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the Bik antibody detected the Bik protein (arrow).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
