BICC1 Antibody

• Catalog Number: orb1248591
• Category: Antibodies
• Description: BICC1 Antibody
• Target: BICC1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-PNSPSIQHISQT
• UniProt ID: Q9H694
• Tested applications: ELISA, IP
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments in Human Brain (Cerebellum), Kidney, Lung and Pancreas lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunoprecipitation:Immunoprecipitation by EB09818 from lysates of HEK293 transfected and overexpressing FLAG-HA-BICC1. Precipitation works best in the presence of SDS. Data kindly provided by anonymous customer.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BICC1, bicaudal C homolog 1 (Drosophila), DKFZp686
• Note: For research use only
• NCBI: NP_001073981.1

HEK293 overexpressing Human BICC1 with N-terminal FLAG, probed with rabbit anti-BCC1 in Western Blot after IP using either orb1248591 or anti-FLAG antibody in the presence or absence of SDS.