Beta-Sitosterol

SKU: orb1304793

Description

Beta-Sitosterol

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 83-46-5
• MW: 414.71
• Purity: 99.95% (May vary between batches)
• Formula: C₂₉H₅₀O
• SMILES: C[C@@]12[C@]([C@]3([C@@]([C@]4(C)C(=CC3)C[C@@H](O)CC4)(CC1)[H])[H])(CC[C@@]2([C@@H](CC[C@H](C(C)C)CC)C)[H])[H]
• Target: Endogenous Metabolite,Lipase,Apoptosis
• Solubility: Ethanol:3.32 mg/mL (8.01 mM);DMSO:Insoluble

Bioactivity

• Target IC50:
  HCT116 cells:140 μmol/mL|KB cells (48 h):13.82 µM|MCF-7 cells (48 h):36 ± 5.1 µM|KB cells (24 h):32.58 µM|PPL:99.99 ± 1.86 μg/mL|MCF-7 cells (24 h):59 ± 3.2 µM
• In Vivo:
  Beta-sitosterol (β-sitosterol) treatment significantly reduced immobility times in mice across all doses tested (10, 20, and 30 mg/kg) in both the Forced Swim Test (FST) and Tail Suspension Test (TST), indicating a notable antidepressant effect. At 30 mg/kg, β-sitosterol's efficacy was comparable to fluoxetine (20 mg/kg) with the most robust effect against the control group (P < 0.001). The percentage decreases in immobility (DID) for FST were 39.27%, 51.23%, and 57.48% for 10, 20, and 30 mg/kg respectively, and for TST were 31.63%, 43.95%, and 53.38% for the same doses, confirming the dose-dependent antidepressant activity of Beta-sitosterol in animal models.
• In Vitro:
  Bioactivity-guided isolation from the hexane fraction of E. indica yielded Beta-Sitosterol (β-sitosterol), Stigmasterol, and Lutein. Beta-Sitosterol and Stigmasterol displayed minimal PPL inhibition, with 2.99±0.80% and 2.68±0.38% at 100 μg/mL (242-243 μM) respectively, compared to higher inhibition by Curcumin (IC50=4.92±0.21 μg/mL) and Quercetin (IC50=18.60±0.86 μg/mL). These compounds showed weaker inhibition than literature values (50% PPL inhibition at 100 μg/mL). Beta-Sitosterol, also extracted from Aloe vera, inhibits the growth of L. donovani promastigotes, implicated in visceral leishmaniasis.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

22,23-Dihydrostigmasterol, b-Sitosterol, BetaSitosterol, Beta-Sitosterol, Betaprost, Azuprostat, Apoptosis, Cupreol, Beta Sitosterol, EndogenousMetabolite, Endogenous Metabolite, β-Sitosterol, SKF14463, SKF-14463, SKF 14463, PPL

Compound Identifiers

PubChem CID

222284