Beta-Sitosterol

SKU: orb1221744

Description

β-Sitosterol has recently been shown to induce G2/M arrest, endoreduplication, and apoptosis through the Bcl-2 and PI3K/Akt signaling pathways. β-Sitosterol, a main dietary phytosterol found in plants, may have the potential for prevention and therapy for human cancer. Although the exact mechanism of action of β-sitosterols is unknown, it may be related to cholesterol metabolism or anti-inflammatory effects (via interference with prostaglandin metabolism). β-Sitosterol induces apoptosis and activates key caspases in MDA-MB-231 human breast cancer cells.(In Vitro):Beta-Sitosterol is one of the most abundant dietary phytosterols. Beta-Sitosterol shows anticancer properties against breast cancer, prostate cancer, colon cancer, lung cancer, stomach cancer, ovarian cancer, and leukemia.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 83-46-5
• MW: 414.72
• Purity: >98% (HPLC)
• Formula: C₂₉H₅₀O
• SMILES: [C@@H]1(O)CC2=CC[C@@H]3[C@@H]([C@]2(CC1)C)CC[C@]1([C@H]3CC[C@@H]1[C@@H](CC[C@H](C(C)C)CC)C)C
• Target: PPL
• Solubility: DMSO : < 1 mg/mL; H2O : < 0.1 mg/mL

Bioactivity

• In Vivo:
  Animal model: Colon cancer rats model. Dosage: 10, 20 mg/kg. Administration: p.o. , suspended in 0.1% CMC (1.0 mL). Result: Reduced the number of aberrant crypt and crypt multiplicity in a dose-dependent manner. Animal model: Streptozotocin-induced hyperglycemia rats model. Dosage: 10, 15, 20 mg/kg. Administration: p.o. Result: Increased insulin levels and decreased HbA1c levels. Improved pancreatic antioxidant levels and decreased LPO levels.
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• In Vitro:
  Cell Cytotoxicity Assay Cell line: MDA-MB-231. Concentration: 16 μM. Incubation time: 3 days. Result: No cytotoxicity at 16 μM Apoptosis Analysis Cell line: MDA-MB-231. Concentration: 16 μM. Incubation time: 5 days. Result: Increased 33% apoptosis when assayed using 1x104 cells and a 6-fold increase in apoptosis when assayed using a smaller number of cells 5x103. Western blot analysis. Cell line: COLO 320 DM. Concentration: 15, 30, 60, 120 μM. Incubation time: 24 h. Result: Decreased β-catenin and PCNA expression.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

β-Sitosterol | 22,23-Dihydrostigmasterol

Quick Database Links

Gene Symbol

PPL