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Catalog Number | orb420283 |
---|---|
Category | Antibodies |
Description | Beta galactosidase antibody |
Species/Host | Gallus |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, WB |
Isotype | IgY |
Immunogen | This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with full length native Beta Galactosidase isolated from E.coli |
Concentration | 1 mg/ml |
Dilution range | ELISA: 1:2,000 - 1:10,000, WB: 1:2,000-1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This Anti-Beta Galactosidase Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E.coli] and anti-Chicken Serum. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays. |
Conjugation | Unconjugated |
UniProt ID | P00722 |
NCBI | NP_414878.1 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | chicken anti-Beta Galactosidase Antibody, chicken Read more... |
Note | For research use only |
Application notes | Anti-Beta-Gal (CHICKEN) Antibody has been tested for use in ELISA and immunoblotting. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. |
Expiration Date | 12 months from date of receipt. |
ELISA, FC, IF, IP, WB | |
E. coli | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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