Recombinant Catenin beta Antibody

SKU: orb699582

Description

Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Research Area

Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways, Wnt

Images & Validation

• Tested Applications: ELISA, FACS, IHC-P
• Dilution Range: Flow cytometry: 0.5-1ug/million cells,ELISA: order Ab without BSA for coating,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant Catenin beta antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rCTNNB1/1507
• Immunogen: A recombinant human beta-Catenin (p120) protein fragment from the human protein was used as the immunogen for the recombinant Catenin beta antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Catenin beta antibody (clone rCTNNB1/1507); Red=isotype control, Blue= recombinant Catenin beta antibody.

IHC staining of FFPE human tonsil with recombinant Catenin beta antibody (clone rCTNNB1/1507). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Catenin beta antibody (clone rCTNNB1/1507) as confirmation of integrity and purity.

Quick Database Links

UniProt

P35222