Beta-actin Antibody (Ascites)

SKU: orb2649354

Description

Beta-actin Antibody (Ascites)

Research Area

Cancer Biology; Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:5000-20000, IHC-P - 1:100-500
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Molecular Weight: 41737 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti-Beta-actin Antibody at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution.Observed band size: 42 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human heart tissue reacted with Beta-actin Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Beta-actin Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-Beta-actin Monoclonal Antibody in HL-60 cell line lysates (35 μg/lane). Beta-actin (arrow) was detected using the purified Mab.

Quick Database Links

UniProt

P60709