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Beta Actin Antibody / ACTB

Catalog Number: orb1823016

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb1823016
CategoryAntibodies
DescriptionAll eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. Alpha-Actin expression is limited to various types of muscle, whereas Beta-Actin and Gamma-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG
ConjugationUnconjugated
ReactivityHuman
ImmunogenA recombinant partial protein sequence (within amino acids 150-350) from the human protein was used as the immunogen for the Beta Actin antibody.
UniProt IDP60709
Tested applicationsIHC-P
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Antibody TypePrimary Antibody
Clone NumberACTB/2370
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis Beta Actin antibody is available for research use only.
NoteFor research use only
Beta Actin Antibody / ACTB

IHC staining of FFPE human spleen tissue with Beta Actin antibody (ACTB/2370). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Beta Actin Antibody / ACTB

SDS-PAGE analysis of purified, BSA-free Beta Actin antibody (ACTB/2370) as confirmation of integrity and purity.

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    100 μg
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