beta 4 Spectrin Antibody: PerCP

• Catalog Number: orb376762
• Category: Antibodies
• Description: Mouse monoclonal antibody against beta 4 Spectrin conjugated to PerCP.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N393/2 (Formerly sold as S393-2)
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000)
• Conjugation: PerCP
• MW: 200kDa
• Target: beta 4 Spectrin
• Entrez: 57731
• UniProt ID: Q9H254
• NCBI: NP_066022.2
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Alternative names: BetaIV-spectrin sigma1 antibody, Non-erythrocytic
• Note: For research use only
• Application notes: A 1:100 dilution of SMC-484 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone N393/2. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) beta 4 Spectrin Antibody (D) Composite.

Western Blot analysis of Monkey COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone N393/2. Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 μg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.