Recombinant B2M Antibody / Beta-2 Microglobulin

SKU: orb639506

Description

Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant B2M antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: B2M/1857R
• Immunogen: Full length recombinant human protein was used as the immunogen for the recombinant B2M antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human bladder carcinoma stained with recombinant B2M antibody (B2M/1857R). Required HEIR: boil tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant B2M antibody (clone B2M/1857R); Red = isotype control, Blue = recombinant B2M antibody.

Western blot testing of human Raji cell lysate with recombinant B2M antibody (clone B2M/1857R). Expected molecular weight: 12-14 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant B2M antibody (clone B2M/1857R) as confirmation of integrity and purity.

Immunofluorescent staining of PFA-fixed human HeLa cells with recombinant B2M antibody (clone B2M/1857R).

Quick Database Links

UniProt

P61769