TRIM3 Antibody

SKU: orb1247733

Description

TRIM3 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 80kDa band observed in Human Brain (Cerebellum) and Rat Brain lysates and approx 85kDa band seen in Mouse Brain (calculated MW of 80.8kDa according to Human NP_006449.2, Mouse NP_001272799.1 and Rat NP_113974.1). Recommended concentration: 0.1-0.3µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: AKREDSPGPEVQP-C
• Target: TRIM3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

TRIM3, BERP, RNF22, TRIM3, tripartite motif-containing 3, HAC1, RNF97, ring finger protein 22, brain expressed ring finger, tripartite motif protein TRIM3, FLJ16135

orb1247733 staining (0.05 ug/ml) of Mouse Brain lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1247733 (0.3 ug/ml) staining of Human Cerebellum (A) and Rat (B) Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

TRIM3

UniProt

O75382

RefSeq (Protein):NP_150594.2, NP_006449.2