BECN1 Antibody

SKU: orb1240118

Description

BECN1 Antibody

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Gallus, Porcine, Rat
• Application Notes:
  Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Beclin-1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Beclin-1.The immunogen is located within the last 50 amino acids of Beclin-1.
• Target: BECN1
• Purification: Beclin-1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Beclin-1 Antibody: ATG6, VPS30, beclin1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein

Western blot analysis of Beclin-1 in A431 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 µg/mL.

Immunocytochemistry staining of A431 cells using Beclin-1 antibody at 1 µg/mL.

Immunofluorescence of Beclin-1 in A431 cells with Beclin-1 antibody at 2 µg/mL.

Quick Database Links

Gene Symbol

BECN1

UniProt

Q14457

RefSeq:AAH10276